DNA

Part:BBa_K3734003:Design

Designed by: Xingjun Zhao   Group: iGEM21_CSU_CHINA   (2021-10-01)


miR21 target


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 52
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use 4XmiR21T at 3'UTR.When we use PCR to clone the fragment, we need to focus on how many times the miR21T repeats.


Source

The miR21T comes from human genome.

References